![]() Here, the labeled cells pass a light beam inside a flow cytometer with high velocity, thereby providing a multitude of information on physical and chemical properties and a lucid quantitative display of different cell populations. ![]() However, when only small amounts of Strep-tagged or biotinylated proteins are present, the unconjugated murine StrepMAB-Classic can be used combined with a secondary antibody of choice.įluorescently stained cells are frequently deployed in flow cytometry analysis, which can be conducted both, before and after cell isolation, delivering insights into cell functions for basic research and clinical trials. Due to their strong and highly specific interaction with Strep-tagged targets, both generate signals with high sensitivity, avoiding the need for a secondary antibody. As viable alternative to antibodies, Strep-Tactin® and Strep-Tactin® XT with different fluorescent conjugates can be used as well. Strep-tagged or biotinylated proteins, StrepMAB-Classic or StrepMAB-Immo conjugated to different dyes can be applied for direct target labeling. While both, chromogenic or fluorescent labels are utilized for detection, fluorescent labeling is especially popular due to the possibility of multicolor staining of different targets. In both methods the sample fixation is essential to preserve the morphology and structure. The advantage of both techniques is the possibility of imaging proteins in their proper histological context, which enables the localization of cellular compartments and can be detected using fluorescence microscopy. In contrast to immunohistochemistry, in immunocytochemistry the focus is not on tissues but on cells. ![]() Immunohistochemistry and immunocytochemistryĪmongst the immunostaining methods, immunohistochemistry is the most prominent one, in which whole tissues can be visualized, and thus, specific targets can be detected.
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